Light-Enhancing Plasmonic-Nanopore Biosensor for Superior Single-Molecule Detection

Wanted to draw your attention to an important paper that Meller group have today on line, in the prestige journal Advanced Materials (Impact factor =19)

The work was carried out by my ex postdoc in the Department (Dr. Ossama Assad) and two of our current PhD students Tal Gilboa and Joshua Spitzberg.

Great publicity for the department, externally, but also internally in the Technion because we are the first to publish something highly significant using the new e-beam lithography system (cost a fortune to Technion…)

Advanced Materials journal is THE #1 journal in material science expect maybe Nature journal .

Light-Enhancing Plasmonic-Nanopore Biosensor for Superior Single-Molecule Detection

Ossama N. Assad, Tal Gilboa, Joshua Spitzberg, Matyas Juhasz, Elmar Weinhold,
and Amit Meller

The development of nanopore-based biosensors has received considerable attention in the past two decades due to their compatibility with a broad range of analytes, including nucleic acids,[1] proteins,[2] and various small molecules.[3] Particularly,nanopore-based DNA sequencing has recently emerged as a viable alternative to sequencing-by-synthesis approaches,[4] offering a highly portable and affordable solution with high throughput and precision.[5,6] Currently the most advanced nanopore-based sequencing methods are based on protein pores, such as the CsgG or MspA channels, which require a ratcheting enzyme to regulate the transport of a DNA strand.[6]  To the full paper click here

plasmonic-nanowell

Figure 1. A plasmonic nanowell–nanopore (PNW–NP) device architecture for enhanced single molecule fluorescence detection.

(a) Schematic cross-section of the PNW-NP device containing a nanowell fabricated in a gold film (orange) with a nanopore drilled in the freestanding SiNx membrane (light green).

(b) Bright-field optical microscopy image (back view) of a nanowell array with 5 μm pitch fabricated on ≈30 μm × 30 μm freestanding SiNx membrane. An “L” shape orientation marker (bright pattern on image) is fabricated on each device to facilitate nanowell identification.

(c) Scanning electron microscopy (SEM) image (top view) of a
typical nanowell with diameter of 120 nm, fabricated in a 130 nm thick polycrystalline Au films.

(d) Transmission electron microscopy (TEM)
image (top view) of a single nanowell with a nanopore drilled in its base. The bright spots in the center (arrow) correspond to the nanopore.

(e) High-resolution TEM image shows a close up view of the drilled ≈4 nm pore.

 

 

 

 

 

 

 

 

To the full paper click here